CD40 (173aa) - Fc Chimera
Symansis" purified CD40 (173aa) - Fchcx Chimera has N-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F. Symansis" CD40 (173aa) - Fchcx Chimera consists of 5-30% carbohydrate by weight. Densitometry of protein isoforms visualized by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein. 1D SDS-PAGE of Symansis" hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation. 1. Eliopoulos AG and Young LS (2004) Curr Opin Pharmacol 4:360-367 |  |
