Cripto-1 - Fc Chimera
Symansis" purified Cripto-1 - Fchcx Chimera hasN- and O-linked oligosaccharides,shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F and a further drop after treatment with a glycosidase cocktail. Symansis" Cripto-1 - Fchcx Chimera consists of 5-30% carbohydrate by weight. Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein. 1D SDS-PAGE of Symansis" hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation. For more details, please see product sheet. 1. Strizzi et al. (2005) Oncogene 24:5731-5741 |  |  |
