EGF R - Fc Chimera
Symansis" EGF R - Fchcx Chimera has N-linked and O-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F and a further drop after treatement with a glycosidase cocktail. Symansis" EGF R - Fchcx Chimera contains 5-30% carbohydrate by weight. Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein. 1D SDS-PAGE of Symansis" hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation. 1. Herbst RS (2004) Int. J. Radiation Oncology Biol. Phys., 59(2):21-26 |  |  |
